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nhs blocking buffer  (Vector Laboratories)


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    Structured Review

    Vector Laboratories nhs blocking buffer
    Nhs Blocking Buffer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhs blocking buffer/product/Vector Laboratories
    Average 93 stars, based on 60 article reviews
    nhs blocking buffer - by Bioz Stars, 2026-03
    93/100 stars

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    Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

    doi: 10.3791/60784

    Figure Lengend Snippet: Materials

    Article Snippet: ​ Name of Material/Equipment Company Catalog Number Comments/Description Alexa Fluor 488 Labeling Kit Invitrogen A30006 Bio-Spin P-6 Gel Columns Bio-Rad 7326221 Biotin Sigma-Aldrich B4501 Use as free biotin in Step 5.6 Biotin-14-dCTP AAT Bioquest 17019 BSA-Biotin Sigma-Aldrich A8549 Coverslips VWR 48393–195 No. 1 ½, 22 × 50 mm dNTP Set Invitrogen 10297018 Float Buoys for Mini Dialysis Device Thermo Scientific 69588 Klenow Fragment (3’→5’ exo-) New England BioLabs M0212S action buffer in Step 2.1.1 and 1X reaction buffer in Step 2.2.2 Lambda DNA New England BioLabs N3011S Mini Dialysis Device Thermo Scientific 69570 10K MWCO, 0.1 mL volume NE Buffer 4 New England BioLabs B7004S NHS-PEG4-Biotin Thermo Scientific 21330 Protocatechuate 3,4-Dioxygenase Sigma-Aldrich P8279 Protocatechuic acid Santa Cruz Biotechnology sc-205818 Silicone Elastomer Kit for PDMS Fabrication The Dow Chemical Company 4019862 Streptavidin Sigma-Aldrich 85878 The Blocking Solution CANDOR Bioscience 110 050 se as casein blocking solution throughout protocol Vinyl Cleanroom Tape Fisher Scientific 19-120-3217 von Willebrand Factor, Human Plasma Millipore Sigma 681300 YOYO-1 Dye AAT Bioquest 17580 0.25 mm Inner Diameter Tubing Cole-Parmer EW-06419–00 25 Gauge Needle Thomas Scientific JG2505X Open in a separate window Materials

    Techniques: Labeling, Lambda DNA Preparation, Blocking Assay